Absorprion Spectrophotometry

THE ABSORPrION , TRANSLOCATION AND ACCUMULATION OF SODIUM IN YOUNG SQUASH

In most plants the concentration of sodium is much lower in the leaves than in the roots. In other species, however, there seems to be an equal distribution of this element between the shoots and the roots. The squash plant, Cucurbita ~, used in the present study is an example of the type of plant that in respect to the roots maintains a relatively low sodium concentration in its leaves. When t...

Fluorescence Spectrophotometry

Fluorescence and phosphorescence are photon emission processes that occur during molecular relaxation from electronic excited states. These photonic processes involve transitions between electronic and vibrational states of polyatomic fluorescent molecules (fluorophores). The Jablonski diagram (Figure 1) offers a convenient representation of the excited state structure and the relevant transiti...

Low-temperature spectrophotometry

Photoreactions of the fourth rhodopsin-like pigment, phoborhodopsin (pR480), of Hulobacrerium halobium were studied by low-temperature spectrophotometry. Upon irradiation of pR480 at -80 as well as 170°C with 436 nm light, a new intermediate having a difference absorption maximum at about 520 nm was produced. We designate this intermediate ‘P520’. Prolonged irradiation caused the formation of a...

Basic UV/Visible Spectrophotometry

Paraffinoma confirmed by infrared spectrophotometry.